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Research in the Thornburg Laboratory is focused on a unique floral organ, the
Nectary.
The nectary is responsible for secretion of floral nectar, the
sweet fluid that is the primary attraction for insect pollinators.
The long term goals of the Thornburg laboratory are to understand
nectary development and function and to be able to manipulate
these functions to achieve improved crop pollination which would
result in higher rates of seed set and thereby, greater yields.
- Understanding nectar composition
| The composition of nectar has been widely studied.
Nectar is an aqueous combination of a variety of substances.
Chief among these are sucrose, glucose, and fructose. At least
11 other carbohydrates have also been identified in nectars of
some flowers. All twenty of the normal amino acids found in protein
have also been identified in various nectars. Other substances
reported in nectar include organic acids, terpenes, alkaloids,
flavonoids, glycosides, vitamins, phenolics, oils, and five proteins.
Our work has focused on the nectar proteins. This work has defined
the Nectar Redox Cycle that functions in protecting the gynoecium
from attack by microorganisms (see publications) |
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- Understanding nectary development
| Many flowering plants produce a rich floral nectar
to attract insect and avian pollinators and thereby increase
their fecundity. Nectar is produced by the nectary, a terminally
differentiated organ often located at the base of the flower.
The mechanisms of floral development that give rise to the nectary
and thence to the floral nectar are completely unknown. |
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- Starch metabolism in the nectary gland
| The nectar of ornamental tobacco is 35%
(by weight) carbohydrate, although the source of carbohydrate
in nectar is unknown. We are investigating the posibility that
nectar sugars derive from starch synthesized in the nectary gland
prior during floral development. Because carbohydrate may be
the most important component of nectar for attracting pollinators,
a clear understanding of the mechanisms required for nectar production
will enable the eventual manipulation of nectar components to
achieve increased yields for insect pollinated crop plants. |
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- Carotenoid metabolism in the nectary gland
| In flower buds, the tissue that will eventually
develop into the nectary gland is photosynthetic (green and contains
chloroplasts). However, during the development of the nectary,
the nectary tissue becomes non-photosynthetic and is filled with
chromoplasts (orange). These studies are designed to understand
the biosynthesis of carotenoids in the nectary gland and simultaneously
to understand the conversion of chloroplasts into chromoplasts.
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